Spring wheat is cultivated early in the spring in moderate climatic areas or cultivated late in the winter when freezing period is over. To understand plant’s response to the cold stress, former studies have led researchers to the identification of several proteins either in wheat or in other plants. In order to achieve further information regarding with the mechanism of spring wheat response to the cold stress at protein expression level, proteomics was applied for an Iranian local spring wheat cv. Kohdasht (LT50=−4 to View the MathML source). Seedlings were first grown in a controlled growth room at View the MathML source for 14 days and subsequently were transferred to View the MathML source (experimental day 0) for 42 days (cold treatment), or they were maintained at View the MathML source (control treatment). Sampling was done on days 0, 21 and 42 of the cold exposure. Protein extraction was carried out and two dimensional analysis were done. Proteins that showed at least two levels of expression changes were identified and mass spectrometry was carried out for their identification. The study led us to the identification of 92 proteins several of which seem to play important role in plant’s response to the cold stress such as cold regulated protein and ferredoxin-NADP(H) oxidoreductase as well as heat shock protein 70. Here we report proteomics analysis of cold responsive proteins and discuss their role in Kohdasht spring wheat cultivar.